Study Strongly Indicates Synthetic/Lab Origin of SARS-CoV-2

Determining origin of CV19 is critical.

To date, we've found no animal indigenous to China capable of transmitting CV19, much less any animal sold in Wuhan's Huanan Seafood Market. Further, the WIV/CCDC Labs just happen to be the world's most active coronavirus repositories. So it is obvious to anyone with a brain CV19 almost assuredly came from a Wuhan Lab. But "almost assuredly" is not quite 100% proof.

As long as the Chinese can claim CV19 was a naturally evolved virus, they can imply that someday we'd discover the animal CV19 originated in.

If however, CV19 is shown to be engineered, there obviously can be no question as to origin. Fauci, Baric, Daszak and their respective labs would be finally, and justifiably excoriated. Fauci and Daszak would be subject to criminal charges.

So this is important work. It is pre-release, awaiting review,and Fauci & Co will do everything in their power to squash it.

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Endonuclease fingerprint indicates a synthetic origin of SARS-CoV-2
Valentin Bruttel1, Alex Washburne, and Antonius VanDongen
Department for Obstetrics and Gynecology, University Clinics of Würzburg, Germany; Selva Analytics, Bozeman, Montana, USA; Department of Pharmacology and Cancer Biology, Duke University, Durham, NC, USA
October 20, 2022

Abstract
To prevent future pandemics, it is important that we understand whether SARS-CoV-2 spilled over directly from animals to people, or indirectly in a laboratory accident. The genome of SARSCOV-2 contains a peculiar pattern of unique restriction endonuclease recognition sites allowing efficient dis- and re-assembly of the viral genome characteristic of synthetic viruses. Here, we report the likelihood of observing such a pattern in coronaviruses with no history of bioengineering. We find that SARS-CoV-2 is an anomaly, more likely a product of synthetic genome assembly than natural evolution. The restriction map of SARS-CoV-2 is consistent with many previously reported synthetic coronavirus genomes, meets all the criteria required for an efficient reverse genetic system, differs from closest relatives by a significantly higher rate of synonymous mutations in these synthetic-looking recognitions sites, and has a synthetic fingerprint unlikely to have evolved from its close relatives. We report a high likelihood that SARSCoV-2 may have originated as an infectious clone assembled in vitro.

Lay Summary
To construct synthetic variants of natural coronaviruses in the lab, researchers often use a method called in vitro genome assembly. This method utilizes special enzymes called restriction enzymes to generate DNA building blocks that then can be “stitched” together in the correct order of the viral genome. To make a virus in the lab, researchers usually engineer the viral genome to add and remove stitching sites, called restriction sites. The ways researchers modify these sites can serve as fingerprints of in vitro genome assembly. We found that SARS-CoV has the restriction site fingerprint that is typical for synthetic viruses. The synthetic fingerprint of SARS-CoV-2 is anomalous in wild coronaviruses, and common in lab-assembled viruses. The type of mutations (synonymous or silent mutations) that differentiate the restriction sites in SARS-CoV-2 are characteristic of engineering, and the concentration of these silent mutations in the restriction sites is extremely unlikely to have arisen by random evolution. Both the restriction site fingerprint and the pattern of mutations generating them are extremely unlikely in wild coronaviruses and nearly universal in synthetic viruses. Our findings strongly suggest a synthetic origin of SARS-CoV2.
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