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L. reuteri ATCC PTA 6475 (probiotic related)

Posted on 5/19/18 at 2:54 pm
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/19/18 at 2:54 pm
This is from the blog from the Dr. who is the author of Wheat Belly.

So I have read about this multiple times but mainly ignored it. I figured I would share with the board. For this to be of any benefit you would need to make your own yogurt from a particular bacterium strain. I ordered all necessary equipment and will embark on this experiment personally to see if the benefits are legit.

I think some of these health benefits if legit probably align with just about everyone's goals here whether you're trying to add muscle, lose fat, or who knows what else.

quote:

Here is a summary on the Lactobacillus reuteri yogurt that I have been talking about over several blog posts, all put together for ease.

I’ve been discussing this idea of making yogurt by starting with a specific strain of Lactobacillus reuteri ATCC PTA 6475, based on the detailed studies conducted at MIT and elsewhere, both experimental animal and human, that have suggested dramatic effects. Those effects include:

Complete shut-down of appetite, an “anorexigenic” effect, that can be used to facilitate intermittent fasting or break a weight loss plateau. This, along with an increase in metabolic rate, explain why weight loss results.

Dramatic increase in skin thickness and skin collagen, along with acceleration of skin healing, a surrogate for overall youthfulness and health. I’m a big fan of dietary collagen, such as those provided by collagen hydrolysates, bone broths/soups, slow-cooking meats, eating the skin on chicken and fish, etc. This L. reuteri strategy amplifies this effect considerably.

Increased oxytocin–A doubling of oxytocin blood levels was observed in mice, the effect responsible for the extravagant skin benefits, reduced insulin resistance, dramatic increases in testosterone in males, increased estrogen in females (magnitude unclear), thicker and more plentiful hair (though the consistency of this effect is not yet clear). Other studies have demonstrated substantial weight loss, especially from visceral fat, increased muscle mass, and increased bone density (protection from osteoporosis/osteopenia).



He goes into the methodology of making it in more detail in the link but:
quote:

So we start with L. reuteri ATCC PTA 6475 provided by the Swedish company, BioGaia, who has somehow locked this species up with patents (not sure how; I thought biological organisms were non-patentable). Their product is called Gastrus and combines the ATCC PTA 6475 strain with the DSM 17938 strain. (Just Google “BioGaia Gastrus” to find a retailer.) Problem: There are only 100 million CFUs (live organisms) per tablet. I have not observed any substantial health benefits by ingesting the tablets.

So I have been amplifying bacterial counts by making yogurt. The counts are further increased by performing fermentation in the presence of prebiotic fibers. Just as ingesting prebiotic fibers increases bacterial counts in your intestines, so it goes in making yogurt, as well.

The yogurt is thick, delicious, and contains a marked increase in bacterial counts, though I have not yet performed a formal count. Given the extraordinary thickness of the end-product, it is likely that trillions of CFUs are present, sufficient to convert the soupy liquid of your starting milk, half-and-half, cream, coconut milk or other starter to rich, thick yogurt, sometimes thick enough to stand up on a plate. People who consume 1/2 cup per day of this preparation (mix with blueberries, strawberries, etc.) are reporting the effects listed above. And this yogurt is so much richer and better tasting than products you buy in grocery stores.


LINK /

I'm going to fit this into my existing routine using the 1/2 cup daily dosage recommended and will report back to see if anything worth noting happens.
Posted by Lazy But Talented
Member since Aug 2011
14452 posts
Posted on 5/19/18 at 5:38 pm to
Lol I just started taking these last week.

I haven't done the yogurt though.
Posted by Junky
Louisiana
Member since Oct 2005
8382 posts
Posted on 5/19/18 at 5:55 pm to
Though I enjoyed his book, probiotics in gut health have too many variables for just one fix. I am not even sure I believe in probiotics because of the variability. Humans can adapt so easily on a variety of diets - so gut health can vary.

Now, I have my own feelings on diet and fixing gut health. The above is a smorgasbord of ideas from multiple angles.
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/19/18 at 6:31 pm to
quote:

Lol I just started taking these last week.

I haven't done the yogurt though.




I meant to put this part in bold but forgot

quote:

There are only 100 million CFUs (live organisms) per tablet. I have not observed any substantial health benefits by ingesting the tablets.
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/19/18 at 6:33 pm to
quote:

hough I enjoyed his book, probiotics in gut health have too many variables for just one fix. I am not even sure I believe in probiotics because of the variability. Humans can adapt so easily on a variety of diets - so gut health can vary.

Now, I have my own feelings on diet and fixing gut health. The above is a smorgasbord of ideas from multiple angles.




I hear ya. I'm not expecting any medical miracle to take place. I figure that it can't really hurt anything so I'm going to give it a try.

On a side note I read the book about 10 years ago. Followed the program for a while with success and got off the wagon.

I'm giving the wheat and grain elimination thing another try since I don't really eat much of it anyway. This is more or less something to keep me busy.
Posted by Lazy But Talented
Member since Aug 2011
14452 posts
Posted on 5/19/18 at 7:52 pm to
I'll hold off on taking the tablets. Report back with your results after you try the yogurt method, please.


Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/20/18 at 11:15 am to
I just got my equipment in. Need to run to the store to get some canning jars and I'll commence the science project this afternoon.
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/22/18 at 8:23 am to
I finished making the yogurt last night and refrigerated in a couple of pint sized jars.

Had some for breakfast this morning. Tasted fine but would probably be better with some blueberries or something. I didn't flavor it with anything.

I'm going to take daily for a couple weeks to see if there if there are any noticeable health benefits as described above.
Posted by Farkwad
Byzantium
Member since Sep 2010
2669 posts
Posted on 5/22/18 at 3:43 pm to
Did it come out more soupy like Kefir or were you able to get it thick?
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/22/18 at 3:45 pm to
It came out thick. I used heavy cream to make it more of a keto yogurt so that may have helped. I've read that for some people the first batch comes out thin but the subsequent batches thicken up. I have a pic of the finished product I'll post in a few minutes
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/22/18 at 3:51 pm to
Brought some to work this morning in a pint sized jar



It's pretty firm. I was worried it might not be firm enough when I finished incubating but after chilling overnight it seemed to get a little firmer.

I'll be interested to see how subsequent batches come out.
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/22/18 at 3:57 pm to
I just took a dollop and put on the plate for better reference



One thing I noticed is this doesn't taste tart like yogurt. It has the consistency of a pretty thick yogurt without the tartness. Looking into the science of it I think it's because I used cream that has less sugar than milk per unit weight/volume. The sugar is turned into lactic acid during the process so that's what gives it the tart flavor.

I'm perfectly fine with it not being tart and with a rather mild taste it should be able to take on whatever flavors I want it to. I'm contemplating making some flavored frozen yogurt out of one of the future batches.
This post was edited on 5/22/18 at 4:15 pm
Posted by Lazy But Talented
Member since Aug 2011
14452 posts
Posted on 5/23/18 at 7:34 pm to
How difficult was it to make? What "equipment" did you need?
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/24/18 at 5:19 am to
Very easy to make

I just got an Anova so I used that for the incubation period

You can also use a yogurt maker or do it on the stove or in the oven. The sous vide machine made sense for me since it keeps a precise temperature for a very long time.

I'll go through the steps I used

1. Mix a small amount of the cream with the crushed tablets and inulin to make a slurry.

2. Whisk in the rest of the cream

3. Heat to 180 F. I did this in the microwave. Takes a little over 6 minutes. Check with an instant read thermometer

4. Wait for it to cool to somewhere around 110-115

5. Pour into jars and seal and set the machine to 110 for 30 hours

6. Refrigerate

I started making a second batch yesterday. Once the first batch is made you can make the subsequent batches with existing yogurt. I did add a tabespoon of inulin again though. Apparently this helps feed the bacteria cultures and is also good for thickness.

I'll advise on how the second batch comes out. Goes in the fridge tonight and I'll have it ready tomorrow.

Posted by Lazy But Talented
Member since Aug 2011
14452 posts
Posted on 5/24/18 at 10:44 am to
Sweet. Let us know whenever (if) you start seeing/feeling of the benefits.

Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/24/18 at 12:57 pm to
Will do

By the way, I know the author of the article said that he didn't see any benefits from taking the tablets orally but I have read reviews of people seeing differences in skin and hair appearance.

Not sure what your end goal was with taking them but if that was one of them there might be a benefit to taking orally.
Posted by tommy2tone1999
St. George, LA
Member since Sep 2008
6781 posts
Posted on 5/26/18 at 9:53 am to
Gut biota is an intersting area of study that’s just getting started. I asked my supplement company why they chose not to use strains of Lactobacillus that had a more dramatic effect on regulating blood sugar or helping with weight loss instead of the strains they chose. Their answer was that while some strains had greater effects on those things, they also were more sensitive to changes in environmental conditions which gave them a shorter shelf life such that most if not all of the population was wiped out before the consumer was able to use it. It could be a BS reason, but it makes sense. All I’m saying is that you should also consider how hardy the species is of probiotic you chose as well as the effects it demonstrated in controlled studies.
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/26/18 at 10:53 am to


quote:

Their answer was that while some strains had greater effects on those things, they also were more sensitive to changes in environmental conditions which gave them a shorter shelf life such that most if not all of the population was wiped out before the consumer was able to use it.

Interesting

Worst case scenario is I'm adding some cultured cream to my diet
Posted by Powerman
Member since Jan 2004
162231 posts
Posted on 5/26/18 at 6:13 pm to
Update: I don't know if it's related to this or something else but energy levels are through the roof.


Dropped 2 lbs this week. Don't know if that's related or not since I've otherwise stayed on my normal routine. Too early to say but it does appear for now to be having some benefit with mood and energy.

I might need to start making bigger batches because it seems like I'm in a cycle of making more every few days.
Posted by Hulkklogan
Baton Rouge, LA
Member since Oct 2010
43300 posts
Posted on 5/27/18 at 7:38 am to
Interesting.

My Dietician has me taking VSL #3 because she thinks I've got leaky gut. It's got like 400billion cultures.. but it's freeze dried and expensive, and I worry about its efficacy. I have been taking it for almost a month and haven't really noticed much difference.

I have started home brewing kombucha, eating sourkraut and Kim chi on top of the probiotic. this yogurt intrigues me too.
This post was edited on 5/27/18 at 8:06 am
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