re: Lunch Thread 7.30.21: Petition for Temp Covid Board Edition

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dukke v

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A big excess of beads with a lot of capture antibodies on them means that your target protein is going to be vacuumed up out of solution really quickly, and as this post explains, that’s a real advantage in these methods. The more time proteins spend rubbing up against and rolling across surfaces, the more degraded and denatured they get, so speed is definitely a virtue here. Once you’ve done this, then (as with an ELISA) you set up a “sandwich” assay format, where your protein of interest ends up captured by one antibody, while its remained exposed surface then gets bound to another detection antibody. This detection antibody has biotin tags already attached to it, and every time you see biotin stuck onto something, you know that it’s going to be used to grab onto some other reagent that has biotin’s lifelong love, streptavidin, attached to it. Indeed, once you’ve hit the sample-incubated beads with the detection antibody, you add in a streptavidin-beta-galactosidase enzyme conjugate, so that in the end, every bead that captured a target protein now has a detection antibody stuck to that in turn, which is then stuck to a streptavidin-enzyme species. A sandwich indeed!

Georgia

taco bell because someone gave my stepson a bunch of gift certificates he doesn't want and I am lazy

all of that is in English

And what’s it supposed to tell me?????

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well now I know you are smarter than me just like slaggy

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no way, Slaggy rules that world here

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And what’s it supposed to tell me?????

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here you go:

The spike protein is located on the outside of a coronavirus and is how SARS-CoV-2 (the coronavirus) enters human cells. Its location on the outside of the virus makes it so the immune system can recognize it easily. The spike protein is unique to SARS-CoV-2 – it doesn't look like other proteins your body makes.Jul 2, 2021

Hey toof

Coc may be limited today. My stomach is bubbling from eating stupid pizza last night.

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Slaggy rules that world here

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I'm barely literate

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I have a metric ton of key limes and other dessert ingredients sitting here

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if you can still taste things and have the energy to do so, I think you should have a weekend pajama dessert binge

you know, for the Vitamin C

grilled shrimp salad
crackers
iced tea