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Posted on 7/16/20 at 1:50 pm to mooseofterror
quote:Odd question. Akin to asking a pilot if he's ever designed an airplane.
have you ever designed, tested and validated a qPCR assay?
False positives in Sars-CoV-19 PCR testing are described as more often indicative of sample contamination, than with flaws in the actual assay. If you have contrary information specific to tests listed, please post it. Here is an example:
quote:
The results of the self-collection validation were consistent with expected results. All positives (36/36) remained positive 72 hours post shipping. No false positives were detected (30/30). All samples had strong Human RnaseP signals indicating all participants were successful in collecting human biological material. Samples shipped without a gel pack (a worst-case shipping condition) showed no change in result for either RnaseP or COVID-19 targets.
Regarding:
quote:Indeed. Hence the "(finally)" in my post about the CDC test. I've posted extensively ( months ago)
The very first qPCR test used by the CDC was laughable and showed such incompetence that we should not trust ANYTHING from the CDC.
about the CDC's initial testing faux pas ( here is another post from March).
Posted on 7/16/20 at 1:56 pm to Blizzard of Chizz
quote:
relying on a test that was hastily developed
Speaking of 'hastily developed' where did the 6-feet study come from that determines the proper amount of social distancing? Did they compare it to 5-foot and 7-foot infection examples?
Or did they just grab the number out of their asses?
Posted on 7/16/20 at 2:00 pm to Zach
quote:
Or did they just grab the number out of their asses?
Like a shite show?
Posted on 7/16/20 at 2:05 pm to BlackHelicopterPilot
quote:Both actually. False PCR positives, if they occur d/t lab error etc., will unnecessarily subject someone to quarantine. Kind of a PITA. But little more. However, a false negative could erroneously unleash an infected person to transmit disease to dangerously vulnerable folks.
Wait...what does this mean? Is it "not much of issue" - meaning it does not happen. Or, "not much of an issue"- meaning only false Negatives are important.
The opposite concern presents with sera AB testing. With AB testing a false positive is more problematic. It that case, the patient is left to assume he's been exposed and is immune with CV19 antibodies when he actually isn't. A false negative AB test leads an immune patient to proceed as if he is still at risk.
Posted on 7/16/20 at 2:42 pm to ChEgrad
quote:Correct.
I read that the PCR will pick up the pieces of virus already destroyed
A positive PCR doesn't assess viability of the shed virus.
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