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re: Negative pcr tests?

Posted on 8/2/21 at 12:35 pm to
Posted by NineLineBind
LA....no, the other one
Member since May 2020
6966 posts
Posted on 8/2/21 at 12:35 pm to
Polymerase Chain Reaction.

It's the technology behind the covid tests.
Posted by mooseofterror
USA
Member since Dec 2012
1339 posts
Posted on 8/2/21 at 12:43 pm to
quote:

Polymerase Chain Reaction.

It's the technology behind the covid tests.


qPCR, Quantitative PCR, Real-Time PCR all the same thing. This method takes a reading after each amplification cycle.

Traditional PCR is an end-point measurement that take the reading at the end of all amplification cycles.

It is also noteworthy that this is the testing method and that assays can be designed to detect any nucleotide sequence, you just need to design flanking primers to your region of interest and in this case an internal primer (probe) for detection.

The big question is, where did they get the reference COVID-19 sequence from? did they isolate and culture the virus in a lab ?? or is it synthetic or generated with a computer?
Posted by cajunangelle
Member since Oct 2012
147463 posts
Posted on 8/2/21 at 6:47 pm to
1 negative.
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