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re: Negative pcr tests?
Posted on 8/2/21 at 12:43 pm to NineLineBind
Posted on 8/2/21 at 12:43 pm to NineLineBind
quote:
Polymerase Chain Reaction.
It's the technology behind the covid tests.
qPCR, Quantitative PCR, Real-Time PCR all the same thing. This method takes a reading after each amplification cycle.
Traditional PCR is an end-point measurement that take the reading at the end of all amplification cycles.
It is also noteworthy that this is the testing method and that assays can be designed to detect any nucleotide sequence, you just need to design flanking primers to your region of interest and in this case an internal primer (probe) for detection.
The big question is, where did they get the reference COVID-19 sequence from? did they isolate and culture the virus in a lab ?? or is it synthetic or generated with a computer?
Posted on 8/2/21 at 1:06 pm to mooseofterror
quote:
The big question is, where did they get the reference COVID-19 sequence from? did they isolate and culture the virus in a lab ?? or is it synthetic or generated with a computer?
Read the other day that they use similar coronaviruses, and something else I no longer remember.
Also, you sure those pcr tests take a reading after each amplication? If so, why don't they report how many amplification cycles it took to reach a positive result? They have that data but just don't report it?
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